ROS-Nitric Oxide Pathway Reagents and Kits
Thermo Scientific™ Peroxidase Suppressor
Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.
Invitrogen™ Total Nitric Oxide Assay Kit
Measure total nitric oxide (NO) as the single value (sum) of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.
Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis
Enables detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent
Novus Biologicals™ GABA Assay Kit (Fluorometric)
for determination of GABA content in serum, plasma, tissue and cell samples.
Abnova™ Nitric Oxide Synthase Assay Kit
For quantitative colorimetric determination of nitric oxide synthase activity at 540nm
Abcam DCFDA/H2DCFDA CELL ROS ASSAY KIT 300 TEST
DCFDA/H2DCFDA CELL ROS ASSAY KIT 300 TEST
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc ROS Assay Kit -Highly Sensitive DCFH-DA- (100 tests) allows high-sensitivity detection of total ROS with fluorescence microscopes, plate readers or flow cytometers (Ex: 505 nm/Em: 525 nm) 100 tests
Reactive oxygen species (ROS) are primarily produced in mitochondria during ATP synthesis. ROS play an essential role in signaling pathways and the immune system, whereas increasing ROS is associated with diseases and cellular senescence. Recent studies suggested that ferroptosis is a new type of cell death characterized by iron dependency and increasing ROS. Thus, ROS detection has been attracting considerable interest in ferroptosis research. DCFH-DA is widely used for ROS detection, but it has some limitations like weak fluorescence signals and high background. Dojindo’s ROS Assay Kit -Highly Sensitive DCFH-DA- overcomes these limitations. The dye employed in the kit allows ROS detection with higher sensitivity than DCFH-DA. Moreover, the Loading Buffer included in this kit maintains cellular health during assays. Dojindo has various reagents for ferroptosis research. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
DOJINDO MOLECULAR TECHNOLOGIES INC ROS ASSAY-PHOTOOXIDATIONRESIST
NC2316000 ROS ASSAY-PHOTOOXIDATIONRESIST
Abcam Cellular ROS Assay Kit (Orange)
Cellular ROS Assay Kit (Orange)
The product is subject to the following: Abcam Restricted Use Statement
Apexbio Technology LLC GST-Colori-Act-Assay-Kit
GST Colorimetric Activity Assay Kit is widely utilized in biochemical and pharmacological research for quantifying glutathione S-transferase (GST) activity Originating from studies focusing on detoxification pathways the assay measures the conjugation of reduced glutathione to a GST substrate resulting in a colorimetric change proportional to enzyme activity This mechanism is key to understanding the role of GST in detoxification and cellular protection against oxidative stress The assay typically demonstrates in vitro activity in the nanomolar to low micromolar range It is commonly applied in cell models and animal studies especially for drug screening to evaluate potential inhibitors or activators of GST Experimental concentrations typically depend on the specific research design allowing flexibility to suit various experimental conditions The kit provides a reliable tool in elucidating GST s physiological and pathological roles
Apexbio Technology LLC Trypsin-Act-Colori-Assay-Kit
Trypsin Activity Colorimetric Assay Kit is designed for the quantification of trypsin activity in biological samples Originating from protease studies this kit employs a colorimetric mechanism where trypsin cleaves a specific substrate releasing a chromophore that can be measured spectrophotometrically Known for its high sensitivity the assay detects activity in the nanomolar to low micromolar range It is commonly used in vitro for enzyme kinetics and inhibitor screening Typical applications include cell models to study protease involvement in various biological pathways animal studies to evaluate physiological roles and drug screening to identify potential protease inhibitors Experimental concentrations typically depend on the specific research design Providing precise quantitative data this kit is an essential tool for researchers characterizing trypsin behavior and its implications in disease and therapeutics
Apexbio Technology LLC AcetoAc-Colori-Assay-Kit
Acetoacetate Colorimetric Assay Kit is a valuable tool in biomedical research primarily utilized to quantify acetoacetate levels in biological samples Originating from the biochemical assessment category this kit operates by enabling colorimetric detection of acetoacetate through its interaction with specific reagents leading to a measurable change in absorbance This change is proportional to the acetoacetate concentration providing insights into metabolic pathways and energy production The assay is frequently used in vitro and exhibits activity in the nanomolar to low micromolar range Typical applications include cell models animal studies and drug screening aiding in understanding metabolic disorders and evaluating therapeutic interventions Experimental concentrations usually depend on the specific research design This kit is integral for researchers exploring ketone metabolism and its implications in conditions such as diabetes and ketosis